The study was conducted on 145 buffaloes brought to Teaching Veterinary Clinical Complex, LUVAS from Hisar and adjoining villages, with signs of anorexia and decreased milk yield. Urine samples from buffaloes were screened for ketosis using two tests (Rothera’s test and Keto-Diastix-strip test). The disease was confi rmed in 24 buffaloes as primary ketosis on the basis of clinical signs (selective anorexia, drastic reduction in milk yield), absence of any other concurrent disease and two urine tests. Comparison of infected was made with eight apparently healthy buffaloes kept as control. Hematological fi ndings in diseased animals revealed anemia, leucopenia, lower mean values of total erythrocyte count (TEC) and packed cell volume (PCV), eosinophilia and monocytosis whereas biochemical fi ndings shows hypoglycemia, hypocalemia, hypoproteinemia hypercholesterolemia, high triglycerides and enhanced alkaline phosphatase activity in affected animals as compared to control group.